- Title Information
- Title
- Leveraging Organoid Models to Study Candida-Host Interactions and Candidalysin-Dependent Damage in Vulvovaginal and Systemic Candidiasis.
- Type of Resource (primo)
- dissertations
- Name:
Personal
- Name Part
- Khalil, Aseel Khalil Hussein
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Bennett, Richard
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Jamieson, Amanda
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Vaishnava, Shipra
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Biotechnology
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2025
- Physical Description
- Extent
- , 45 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2025
- Genre (aat)
- theses
- Abstract
- Given the widespread nature of vulvovaginal candidiasis, understanding the
molecular mechanisms underlying its pathogenesis and systemic spread could inform
therapeutic development. This study uses vaginal and colon epithelial organoid models
to assess transcriptional and inflammatory responses of mammalian hosts to Candida
albicans and its secreted toxin, candidalysin. Due to the limitations of three-dimensional
organoids in examining infectious disease, we optimize their use for the study of
Candida-host interactions by generating vaginal epithelial organoid-derived monolayers.
RNA-seq analysis reveals an upregulation in hypoxic-stress that was corroborated by a
perturbation of oxygen consumption rates following infection with C. albicans within the
vaginal epithelia. Through a variety of in vitro cell viability assays, we show that
candidalysin facilities adhesion, cell death, and can compromise barrier integrity. This
data highlights the potential translational role of Candidalysin as a therapeutic target in
the treatment of candidiasis and the appeal of organoid models in identifying novel
respiration-related phenotypes.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00832383")
- Topic
- Biology
- Subject
- Topic
- infectious disease
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20250707