- Title Information
- Title
- Optimizing T cell/Antigen Presenting Cell coculture conditions for analysis of signaling from the T cell receptor and Major Histocompatibility Complex
- Type of Resource (primo)
- dissertations
- Name:
Personal
- Name Part
- Hildebrandt, Tobias Robert
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Salomon, Arthur
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Horrigan, Diana
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Marshall, John
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Biotechnology
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2023
- Physical Description
- Extent
- vi, 17 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2023
- Genre (aat)
- theses
- Abstract
- Abstract of Optimizing T cell/Antigen Presenting Cell coculture conditions for analysis of signaling from
the T cell receptor and Major Histocompatibility Complex, by Tobias Robert Hildebrandt, Degree ScM.,
Brown University, May 2023.
T-cell receptor ligation of peptides displayed on the Major Histocompatibility Complex (MHC) of
Antigen Presenting Cells (APC) initiate signaling events. Several studies reported canonical signaling events
upon engagement of the T-cell receptor complex, yet MHC reverse signaling remains understudied. Here, we
describe an optimized approach to permit the study of proximal signaling in live T-cells and APCs simul-
taneously via coculture of CD8+ Jurkat T-cells expressing an OT-1 receptor capable of ligating Ovalbumin
with a B-cell X T-cell hybrid expressing an empty MHC capable of binding to Ovalbumin in solution. A
protein of the MAPK/ERK pathway was used as readout for early signaling. It was shown that only in
the presence of Ovalbumin, T-cells with an OT-1 receptor and cells with an empty MHC Complex can fully
activate T-cell signaling. Methods such as the one described in this body of work permits the simultaneous
study of proximal signaling events in live APCs and T-cells, allowing for the characterization of reverse
MHC signaling in different cell types. This method also permits the study of signaling events unique to
cell therapies, including signaling in Chimeric Antigen Receptor T-cells (CAR-T), in an in vitro model that
closely resembles the human’s body biology.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01141544")
- Topic
- T cells
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00850288")
- Topic
- Cellular signal transduction
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01006236")
- Topic
- Major histocompatibility complex
- Subject
- Topic
- Parameter Optimization
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20230602