- Title Information
- Title
- Cartilage Derived Progenitor Cells Exhibit Paracrine Effects that Direct the Healing Process
- Type of Resource (primo)
- dissertations
- Name:
Personal
- Name Part
- Lin, Yu Ting
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Jayasuriya, Chathuraka
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Wei, Lei
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Fleming, Braden
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Department of Molecular Pharmacology, Physiology and Biotechnology
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2022
- Physical Description
- Extent
- vii, 33 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2022
- Genre (aat)
- theses
- Abstract
- Meniscal tearing is one of the most common types of orthopedics injuries and found in people of all ages. Meniscal tearing is also a risk factor of knee osteoarthritis. Tears that occur in the inner white zone of the meniscus, which has no vascularity, presents a significant clinical challenge due to intrinsically poor healing capacity of this region. Mesenchymal stem/progenitor cell (MSC)-based repair is an emerging and effective preclinical strategy that is being explored to augment and ultimately improve clinical approaches that are commonplace for treating these injuries. Current research involving exogenous cell-based meniscus tissue repair and regeneration utilizes cells from various tissue sources, including bone marrow, adipose (fat), synovium, and cartilage. As a phenotypically similar connective tissue to meniscal fibrocartilage, articular cartilage derived progenitor cells (CPCs) have been used to stimulate meniscal tissue healing. It has been reported that CPCs have elevated expression of the transcription factor SOX9, which is central for chondrogenic matrix synthesis. However, the way CPC interact with native meniscal fibro chondrocytes to promote their cell proliferation and tissue remodeling to aid in meniscal healing remain unknown. This study examines the role of extracellular vesicles (EVs) as a potential mechanism for the observed cell mediated anabolic effects of CPCs. Our results show that CPCs do produce EVs under normal conditions. Conditioned media collected from CPC cultures stimulated increased meniscal cell migration. Further, co-culturing CPCs with meniscal fibro chondrocytes directly regulate expressions of SOX9 and aggrecan in the meniscal cells. Overall, this study provides insight into the mechanism of action of this promising progenitor cell population, which can play an active role in the cartilaginous tissue repair.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01763304")
- Topic
- Mesenchymal stem cells
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00817187")
- Topic
- Articular cartilage
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01016164")
- Topic
- Meniscus (Anatomy)--Wounds and injuries
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01132975")
- Topic
- Stem cells
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20220706