- Title Information
- Title
- Characterization of Nucleic Acid Delivery Vehicle Products using Microfluidic Concepts
- Type of Resource (primo)
- dissertations
- Name:
Personal
- Name Part
- Coll De Peña, Adriana
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Tripathi, Anubhav
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Darling, Eric
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Shukla, Anita
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Hurt, Robert
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Wong, Ian
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Goswami, Somdatta
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Bailey-Hytholt, Christina
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Biomedical Engineering
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2024
- Physical Description
- Extent
- xxxi, 254 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Ph. D.)--Brown University, 2024
- Genre (aat)
- theses
- Abstract
- In recent years, there has been an exponential growth in biopharmaceutical development stemming from its ability to treat previously incurable diseases and to go from pathogen discovery to vaccine creation at unprecedented speeds, as highlighted by the COVID-19 response. Broadly defined as biological pharmaceuticals manufactured using biotechnology, the manufacturing of biopharmaceuticals requires multiple steps of purification and validation that significantly increase the production cost. The validation of the production and different purification steps requires the integration of analytical monitoring throughout the process. However, despite being developed and suitable for the less complex chemical pharmaceuticals, analytical tools have not been capable of evolving at the same rate as the biopharmaceutical field. This mismatch in evolution has left a significant unmet need for rapid, high-throughput analytical methods capable of analyzing the numerous samples generated during the iterative manufacturing of biopharmaceuticals. With its rapid turnaround times, high-throughput compatibility, and low sample requirements, microfluidic electrophoresis presents a promising solution to address this need. Overall, the work described in this thesis increases the understanding of the electrophoretic transport of biopharmaceutical products to inform the development of streamlined microfluidic analytical methods for the purity assessment of samples. To achieve this, we combine a fundamental understanding of the biochemistry and biophysical properties of each sample and exploit these properties to achieve differentiation. The impact of this work includes increasing the electrophoretic understanding of biopharmaceuticals to decrease the analytical burden of their characterization by reducing personnel-intensive steps, turnaround time, reagent and sample volume requirements, and cost through the use of microfluidics and electrophoresis. The application of this approach was shown with a wide range of nucleic acid delivery vehicle products ranging from nucleic acids to lipids and viruses. Ultimately, we developed translatable technology applicable to numerous biopharmaceutical platforms by investigating the fundamental electrophoretic properties of a wide range of molecules.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00832568")
- Topic
- Biomedical engineering
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01736696")
- Topic
- Microfluidics
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00907684")
- Topic
- Electrophoresis
- Subject
- Topic
- Biopharmaceuticals
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20240126