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Genotyping of OPN3 in Knockout & Wild-Type Mice

Description

Abstract:
Over the course of the summer, I have been looking into several different genotyping protocols that will allow me and the lab to genotype mice for the OPN3 gene. The lab has been producing mice with either a Knockout gene (KO), a Wild-Type gene (WT), or both (heterozygous). My job this summer has been to ultimately contribute to genotyping all of the mice in the lab. However, the protocol that has been used in this lab prior to my arrival has recently stopped working, so I have been spending the summer figuring out what needs to be fixed/replaced and what materials/supplies to use. I have tested several different genotyping kits and protocols, and I am close to finding out what works best for this lab. If I do not have time to help genotype the mice, I hope to contribute by finding the best protocol and kit so that the lab can use this method in the future.

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Citation

Stefan S Atanasov, "Genotyping of OPN3 in Knockout & Wild-Type Mice" (2021). Summer Research Symposium. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/vvwr-7218

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Collection:

  • Summer Research Symposium

    Each year, Brown University showcases the research of its undergraduates at the Summer Research Symposium. More than half of the student-researchers are UTRA recipients, while others receive funding from a variety of Brown-administered and national programs and fellowships and go …
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