- Title Information
- Title
- OPTIMIZING LYOPHILIZATION TECHNIQUES FOR ENHANCED STABILITY AND FUNCTIONALITY OF HYPER-COMPLIANT MICROPARTICLES
- Type of Resource (primo)
- dissertations
- Name:
Personal
- Name Part
- Calderon, Stephanie
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Darling, Eric
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Mathiowitz, Edith
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Huang, Eric
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Biotechnology
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2025
- Physical Description
- Extent
- 12, 25 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2025
- Genre (aat)
- theses
- Abstract
- Microparticles have been researched for their use as a drug delivery system due to
their non-toxicity and flexibility. Recently, advancements have been made to formulate
microparticles with the elasticity and size of mammalian cells, allowing for a wider breadth
of use. The goal of this study was to evaluate different lyophilization techniques and how
they would affect the size and remaining count of hyper-compliant microparticles. We also
observed how lyophilization would affect the drug release profile of a double encapsulated
hyper-compliant microparticle with curcumin inside. All formulated microparticles were
visualized using microscopy and measured using ImageJ. Aliquots from H20 sinks were
taken at predetermined time points and analyzed over 96-192 hours for drug concentration.
Regardless of freezing method, it was found that hyper-compliant microparticles postlyophilization were always smaller than the control, and a significant loss of microparticles
was also associated with lyophilization. With the addition of a surfactant, and sugar,
lyophilization did not have a significant effect on size, but less microparticles were still
counted. Lyophilized double encapsulated microparticles were found to have a slightly
faster dissolution than non-lyophilized double encapsulated microparticles. However, even
by the end of the 192 hours, not all the drug had been release. It was also found that
lyophilization changes the drug release profile of the microparticle from zero order
transport to Fickian diffusion. These findings show that lyophilization of hyper compliant
microparticles can be done in a way that preserves the size of the microparticles without
changing their mechanical properties significantly. Furthermore, it shows that double
encapsulated microparticles may be a viable system as a microcarrier.
- Subject
- Topic
- microparticles
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00934141")
- Topic
- Freeze-drying
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20250707