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Year:: 2023
Contributor:: Rezk, Anna (Author); Garcia, Geronimo (Author); Williams, Blair (Author); Marquez, Josue (Author); Born, Christopher (Author); Garcia, Dioscaris (Author); Brown University. Alpert Medical School. Scholarly Concentration Program. Non-Scholarly Concentrator (research program)
Subject:: Orthopedics; Anti-infective agents; Orthopedic implants

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Abstract:: The emergence of pan-resistant and multi-drug resistant bacteria, due to poor stewardship and the overuse of antibiotics, necessitates the development of new antimicrobials. However, the static progression and discovery of new antibiotics warrants an investigation into novel antibiotic-independent antimicrobials, specifically in the form of silver carboxylate (AgCar) released via a titanium dioxide/polydimethylsiloxane (TiO2-PDMS) matrix. While other silver formulations have been used as antimicrobials for centuries, AgCar in TiO2-PDMS has emerged as a promising formulation due to its matrix chemistry which allows for controlled silver release, thereby minimizing human cell cytotoxicity. This study establishes AgCar’s ability to generate reactive oxygen species (ROS) and decrease catalase production in multi-drug resistant, Gram negative Serratia marcescens and in MSSA. In S. marcescens, a 1x-30x concentration of AgCar resulted in the production of double the amount of ROS when compared to the cell blank (p< 0.05). In MSSA, a 1x-150x concentration of AgCar produced a 3-fold increase in ROS production when compared to controls. In both species, these findings were accompanied by an overall decrease in catalase activity when compared to cell blank. Given the ROS-generating microbicidal mechanism of action of AgCar TiO2-PDMS, it has the potential to be utilized as an antimicrobial, especially synergistically with antibiotics.
Notes:: Scholarly concentration: Non-Scholarly Concentrator

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Citation

Rezk, Anna, Garcia, Geronimo, Williams, Blair, et al., "Characterization of Silver Carboxylate’s (AgCar) Microbicidal Mechanisms in Multi-Drug Resistant Serratia marcescens and Methicillin-sensitive Staphylococcus aureus (MSSA)" (2023). Warren Alpert Medical School Academic Symposium. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:zs7fe9qf/

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Warren Alpert Medical School Academic Symposium

The Warren Alpert Medical School Academic Symposium is an annual event at Warren Alpert Medical School of Brown University that provides Year II medical students a venue to present their summer research in a poster format. Participation in the Symposium …
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Characterization of Silver Carboxylate’s (AgCar) Microbicidal Mechanisms in Multi-Drug Resistant Serratia marcescens and Methicillin-sensitive Staphylococcus aureus (MSSA)