An examle of freely moving Cacna1hChR2eYFP+/- animal following two independent surgical procedures for brain and spinal cord window implantations (procedure VI in Fig. 7). At …
Bioluminescence imaged using BLmini v2.0 in an anesthetized wild-type animal with the cortical application of luciferin (hCTZ). Bioluminescent calcium indicator was expressed via intracortical injection …
Bioluminescence imaged using BLmini v2.0 in an anesthetized wild-type animal with the cortical application of luciferin (hCTZ). Bioluminescent calcium indicator was expressed via intracortical injection …
An examle of freely moving Trpv1ChR2eYFP+/-animal following two independent surgical procedures for spinal cord and brain window implantations (procedure VI in Fig. 7). At the …
Almost 30 years after identifying the genetic mutation underlying Huntington’s disease (HD), treatments remain limited to managing late-stage symptoms of motoric, psychiatric, and cognitive deficits. …
Chemogenetic modulation of neuronal populations allows for precise control over cells engineered to express an exogenous receptor that responds exclusively to specific molecules. Chemogenetic approaches …
In BioLuminescent OptoGenetics, a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. Here, we leveraged this coupling …
The malformation of neuronal circuitry in the developing neocortex leads to a variety of psychiatric disorders later in the organism’s life. By investigating alterations in …
To understand how groups of neurons participate in neural circuits underlying behavior, technologies are needed for observing and controlling the ensembles employed during behavior across …
Bioluminescent optogenetics (BL-OG) uses light emitted from a luciferase in the presence of its luciferin to activate light sensing molecules, including channelrhodopsins and light-sensitive transcription …
Patterns of neuronal activity during early development are believed to guide the assembly of neural circuits. Thus, alterations of neuronal activity in this time window …
Interluminescence is a method for selective modulation of synaptic communication between genetically defined partners. Bioluminescent light from a pre-synaptic released luciferase activates a post-synaptic localized …
Using neuroscientists' tools of the trade one can image in independent animals either the brain or the spinal cord. Yet understanding many biological processes such …
Despite ever improving treatment methods, there still is no way to effectively restore function after spinal cord injury. A promising approach is to re-engage spinal …
Bioluminescent Optogenetics (BL-OG) is a novel approach for controlling neuronal activity in which light-sensing opsins are controlled by “biological light.” Luciferases, enzymes that produce bioluminescent …
State-of-the-art imaging tools rely on a restrained preparation, limiting natural behavior while measuring cellular activity via fluorescence (FL). Recently, advances in wearable microscopy expanded the …