Description
- Abstract:
- Over the last two decades there has been an overwhelming amount of scientific research aimed at furthering our understanding of the brain and neurodegenerative diseases. We know that neural function depends on intricate cascades of intercellular communication and molecular signaling across space and time, yet a comprehensive understanding of the subcellular organization underlying larger complex neural processes remains difficult to capture. One of the biggest hurdles in furthering our understanding of these complex cellular processes is a lack of visual access to the brain and development of imaging platforms capable of capturing dynamic system behavior at the cellular level. However, the development of live imaging techniques including high-resolution microscopy and advanced fluorescent probes has provided valuable information regarding highly complex and multidimensional cellular processes. The experiments outlined in this thesis work explore a proposed methodology which builds upon the advantage of high spatial resolution imaging and the effectiveness of fluorescent probes to better understand multicellular neural dynamics. The central goal of this research is to develop a real-time imaging platform for longitudinal visualization of dynamic cellular morphology and cell-cell interactions amongst neurons, astrocytes, and microglia. Using virally mediated expression of fluorescent reporter proteins in three key cell types (neurons, astrocytes and microglia), this thesis work presents an in vitro model for real time capture of dynamic cellular behavior in cultured three-dimensional primary cortical microtissues.
- Notes:
- Thesis (Sc. M.)--Brown University, 2020
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Citation
Brown, Sophie,
"NeuroBorealis: Live cell imaging of 3D cortical microtissue via AAV-induced fluorescence"
(2020).
Biomedical Engineering Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://doi.org/10.26300/wjt3-j878
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Biomedical Engineering Theses and Dissertations
Theses and Dissertations for the Biomedical Engineering department....