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Code from "Inducing vascular chemotaxis for inosculation with growth factor release and patterned vessels"

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Overview

Year:
2020
Contributor:
Kant, Rajeev J. (Author)
Bare, Colette (Author)
Coulombe, Kareen L. K. (Author)
Subject:
tissue engineering
patterned vessels
inosculation
aortic ring
growth factor release
chemotaxis
DOI
https://doi.org/10.7910/DVN/JE3IYD

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Abstract:
Designing and fabricating engineered tissues for translational applications in regenerative medicine require vascular networks to deliver oxygen and nutrients rapidly to implanted cells. A limiting factor in in vivo translation is the rapid and successful inosculation, or connection, of host and implanted vascular networks and subsequent perfusion of the implant. Here we use a novel platform based on the aortic ring assay for studying pre-inosculative outgrowth of native and engineered vascular networks in a controlled, defined environment, and introduce robust tools for evaluating outgrowth. We demonstrate the creation of engineered, arteriole-scale vessels which are lined by endothelial cells, secrete basement membrane, exhibit tight junctions, and actively sprout into the surrounding hydrogel. Vessel-containing constructs are co-cultured adjacent to aortic rings and the resulting cellular outgrowth is quantified. Native vascular wall cells migrate preferentially towards constructs containing engineered vessels with faster kinetics versus control (no endothelial cells and growth factor-reduced culture medium), and histological evaluation shows increased cellular density and greater network formation. Short-term growth factor release from constructs with non endothelialized channels and in reduced factor medium also stimulatesmsustained vascular outgrowth distance, cellular density, and network formation, akin to engineered vessels in EGM-2 media. In conclusion, we demonstrate comparable effects of engineered vessels in growth-factor rich medium and non-endothelialized channels with growth factor release on vascular morphogenesis and pre-inosculative outgrowth through quantitative metrics of heterotypic cellular outgrowth from rat aortic rings.
Notes:
This research was funded by the National Heart, Lung, and Blood Institute (NHLBI) of the National Institutes of Health (NIH) under award NIH R01 HL135091

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This work is licensed under a Creative Commons Attribution 4.0 International license (CCBY)

Citation

Kant, Rajeev J., Bare, Colette, and Coulombe, Kareen L. K., "Code from 'Inducing vascular chemotaxis for inosculation with growth factor release and patterned vessels'" (2020). Brown University Open Data Collection. Brown Digital Repository. Brown University Library. https://doi.org/10.7910/DVN/JE3IYD

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