Description
- Abstract:
- Using mRNA-Seq and de novo transcriptome assembly, we identified, cloned and characterized nine previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from avGFP. Among these FPs are the brightest GFP homolog yet characterized and a reversibly photochromic FP that responds to UV and blue light. Beyond green emitters, Aequorea species express purple- and blue-pigmented chromoproteins (CPs) with absorbances ranging from green to far-red, including two that are photoconvertible. X-ray crystallography revealed that Aequorea CPs contain a chemically novel chromophore with an unexpected crosslink to the main polypeptide chain. Because of the unique attributes of several of these newly discovered FPs, we expect that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing.
- Notes:
- The NeuroNex Technology Hub is supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352.
Access Conditions
- Use and Reproduction
-
- This work is licensed under a Creative Commons Attribution 4.0 International License
- Rights
- In Copyright - United States
- Restrictions on Use
- Collection is open for research.
Citation
Shaner, Nathan C.,
"Imaging data for Lambert et al. 'Aequorea’s secrets revealed: new fluorescent proteins with unique properties for bioimaging and biosensing'"
(2020).
NeuroNex Data and Research Products, Brown University Open Data Collection.
Brown Digital Repository. Brown University Library.
https://doi.org/10.26300/4x48-y393
Relations
Collections:
-
NeuroNex Data and Research Products
This collection contains publicly available research products, including data, codes, and publications, among others. The NeuroNex Technology Hub is supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352.... -
Brown University Open Data Collection
This collection contains open and public access data sets created by Brown University faculty and student researchers. Increasingly, publishers and funders are requiring that protocols, data sets, documentation and code underlying publications be retained and preserved in repositories, their locations …...