Description
- Abstract:
- Poly (lactic-co-glycolic acid) (PLGA) has been widely used as a biomaterial in regenerative medicine due to its good biocompatibility and biodegradability properties. Moreover, nanotopographies have been shown to play an important role in mediating cellular behavior and tissue responses. Therefore, for the first time, lung/breast cancer/healthy cell functions on PLGA nanometer surface features were systematically investigated in this dissertation. The central hypothesis of this dissertation is that due to altered surface properties, specific PLGA nanopatterns could decrease cancer cell functions whiling increasing healthy cell functions and, thus, could be used as anti-cancer implants after tumor removal. To verify this hypothesis, biological responses of cancer and healthy cells on various nano-smooth and nanopatterned PLGA surfaces with altering surface properties were investigated in vitro. Specifically, lung/breast cancer/healthy cell functions (including adhesion, proliferation, apoptosis and vascular endothelial growth factor (VEGF) secretion) were investigated on various nano-smooth and nanopatterned PLGA surfaces. The 23 nm patterned PLGA showed anti-cancer properties by decreasing VEGF secretion and increasing apoptosis of lung/breast cancer cells. Moreover, chemical modification of nanopatterned PLGA surfaces by using a layer by layer (LBL) self assembly method (specifically, alginate and chitosan monolayers) was reported. The impact of PLGA surface chemistry-induced property alteration on cancer and healthy cell proliferation and VEGF secretion was investigated. The results indicated that alginate-terminated nanopatterned PLGA exhibited optimal anti-cancer properties. Lastly, various surface properties (including roughness, surface chemistry, surface charge, surface wettability and surface free energy) of nanopatterned (also chemically modified) PLGA surfaces was described to understand the role nanotopographies play in mediating cancer cell functions and the factors behind the observed biological responses to biomaterials. More importantly, protein adsorption (fibronectin and vitronectin) was reported to elucidate the way surface topography mediates cell responses. In summary, these results provided the first insights into understanding the role PLGA nanotopography may play in inhibiting cancer cell functions and provided guidance to design ideal biomaterial interfaces for specific purposes.<br/> <br/> <br/> <br/>
- Notes:
- Thesis (Ph.D. -- Brown University (2012)
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Citation
Zhang, Lijuan,
"Nanopatterned PLGA for Anti-cancer Implant Applications"
(2012).
Chemistry Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://doi.org/10.7301/Z0Q23XK6
