Development of Hydrogel Systems for pH Regulation of Cancer Tumor Microenvironment

Trivedi, Shruti

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Year:: 2021
Contributor:: Trivedi, Shruti (creator); Srivastava, Vikas (Advisor); Brown University. School of Engineering (sponsor)
Genre:: theses
Subject:: Cancer; Hydrogel; Sodium bicarbonate
Extent:: ix, 53 p.

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Description

Abstract:: This study describes the development of bicarbonate modified Gellan and PEG hydrogels for the purpose of regulating the pH of its surrounding solution. These hydrogels were tested for their ability to regulate an engineered acidic tumor microenvironment by manipulating its pH level and bringing it closer to the physiological pH of 7.2. Additionally, an analysis of the effect of sodium bicarbonate on mechanical integrity, swelling kinetics, and cell viability was done, while investigating the network parameters for swollen hydrogels using modified Flory-Rehner solution theory. Bicarbonate increased the porosity in PEG hydrogels, thereby contributing to a lower gel stiffness and higher swelling. An inverse trend was observed with Gellan hydrogels due to possibly enhanced crosslinking caused by the presence of Na+ ions. An invasive breast cancer cell line, MDA-MB-231, was tested for proliferation and percentage viability to assess both the efficacy of bicarbonate treatment and the biocompatibility of PEG hydrogels in vitro. The findings of this study establish a promising avenue into the development of a simple, effective, and biocompatible therapeutic using bicarbonate-modified hydrogels for cancer treatment.
Notes:: Thesis (Sc. M.)--Brown University, 2021

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Citation

Trivedi, Shruti, "Development of Hydrogel Systems for pH Regulation of Cancer Tumor Microenvironment" (2021). Engineering Theses and Dissertations. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:2x9tk9q3/

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Engineering Theses and Dissertations

Theses and Dissertations for the Engineering department.
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