Description
- Abstract:
- Understanding nanoparticle absorption is a critical step to improving drug delivery techniques. Current methods to determine nanoparticle absorption are often complex and involve direct analysis through complex imaging techniques. To overcome these barriers, a new method was proposed. Using Infrared Spectroscopy, ex vivo polymeric nanoparticle absorption was identified as changes in absorption peak intensities when compared to control spectra. Polystyrene nanoparticle absorption was detected and further quantified through calibration curves. By correlating polymer concentration with isolated peak ratios, indirect quantification of absorption was achieved. Utilizing the calibration method, factors that affect polystyrene absorption, including particle size, location within the GI, and the presence of the loose mucus, were further examined. Polystyrene nanoparticle and microparticle absorption varied between regions of the GI. In addition, polystyrene appeared to penetrate the loose mucus through passive absorption mechanisms alone. Tertiary, in vivo results remained relatively inconclusive, indicating a need for further studies into polystyrene absorption.
- Notes:
- Thesis (Sc. M.)--Brown University, 2017
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Citation
Perez-Rogers, James Christopher,
"Designing a Novel Approach to Quantify Polymeric Nanoparticle Absorption"
(2017).
Biomedical Engineering Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://doi.org/10.7301/Z0M32T7H
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Biomedical Engineering Theses and Dissertations
Theses and Dissertations for the Biomedical Engineering department....