Adipose-derived stem cell microtissues during mesenchymal condensation and chondrogenesis

Gutierrez, Robert Alejandro

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Overview

Year:: 2021
Contributor:: Gutierrez, Robert Alejandro (creator); Morgan, Jeffrey (Reader); Wong, Ian (Reader); Mathiowitz, Edith (Reader); Darling, Eric (Advisor); Brown University. Biology and Medicine: Biomedical Engineering (sponsor)
Genre:: theses
Subject:: adipose derived stem cells; Microtissues; 3D Cell Culture; cellular forces
Extent:: 14, 95 p.

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Abstract:: Adipose-derived stems cells (ASCs) have key properties such as self-renew and differentiation that makes them a prime candidate to study various issues. An ASC spheroid culture system offers a more physiological environment as it allows for cell–cell and cell–extra cellular matrix interaction. Through these adhesions, cells communicated with each other through biochemical and biophysical signals. This allows for cells to form their shape, their motility, their growth, and even their death. Large spheroid cell culture system do tend to have its limitations as they develop a necrosis core do to oxygen diffusion limitations. Therefore, the need to develop a smaller spheroid system that would allow for high-throughput testing is key In thesis. Smaller cell number spheroid system allowed for the measurement of cellular forces within this system. As cellular mechanics plays a crucial role in this developmental stage as forces exerted on cells by their surrounding environment are dynamic in nature. This makes the cells to continuously re-evaluate their status within the surrounding environment and just accordingly to it. Therefore, mechanical forces an essential element to early tissue formation. The need to learn more about how the surround micro environment play an important role in cell. This poses a problem as there is only a few techniques that have tried to measure these actions but have not looked at the early stages of development. We created a hyper compliant micro particle that was sensitive enough to measure micro environmental forces during mesenchymal condensation. Equality import has been the chondrogenesis response of ASCs at this low cell number. That would allow for high-throughput testing were cellular forces can be measured using chondrogenic spheroid to test chemical screenings. Thus widening the possibilities for highly array format experimental designs
Notes:: Thesis (Ph. D.)--Brown University, 2021

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Citation

Gutierrez, Robert Alejandro, "Adipose-derived stem cell microtissues during mesenchymal condensation and chondrogenesis" (2021). Biomedical Engineering Theses and Dissertations. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:nqcy4jex/

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Biomedical Engineering Theses and Dissertations

Theses and Dissertations for the Biomedical Engineering department.
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