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Bioluminescence imaged using BLmini v2.0 in an anesthetized wild-type animal with the second administration and cortical application of luciferin (hCTZ) from Towards a Brighter Constellation: Multi-Organ Neuroimaging of Neural and Vascular Dynamics in the Spinal Cord and Brain

Description

Abstract:
Bioluminescence imaged using BLmini v2.0 in an anesthetized wild-type animal with the cortical application of luciferin (hCTZ). Bioluminescent calcium indicator was expressed via intracortical injection of AAV2/9-hSyn-C1-mNG-CaBLAM (APD3A6) in the barrel cortex. During this imaging session animal was receiving stimuli of vibrissa. Imaging was performed at 5 FPS. Images were normalized to baseline preceding the administration of hCTZ and variations in intensity reflect percentage change. The data from this experiment is presented in Fig. 12b. The animal received 3 intracortical administrations of hCTZ during this experiment and this video demonstrates second administration.
Notes:
The NeuroNex Technology Hub is supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352.

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Citation

Celinskis, Dmitrijs, "Bioluminescence imaged using BLmini v2.0 in an anesthetized wild-type animal with the second administration and cortical application of luciferin (hCTZ) from Towards a Brighter Constellation: Multi-Organ Neuroimaging of Neural and Vascular Dynamics in the Spinal Cord and Brain" (2024). NeuroNex Data and Research Products, Brown University Open Data Collection. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/w24w-g388

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