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Items (61-80) out of 87 results

Movie: Calcium dependent bioluminescence when applying coelenterazine systemically through lateral ventricle and a calcium agonist directly in cortex (1/2)

Description:
This is a movie showing Calcium Dependent Bioluminescence in a mouse expressing LMC4 in left primary somatosensory cortex. Water soluble Coelenterazine (CTZ) was injected in …
Year:
2017
Contributor:
Gomez-Ramirez, Manuel (Creator)
Subject:
bioluminescent optogenetics
BL-OG
coelenterazine
somatosensory cortex
LMC4
lumicampsin
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Movie: Calcium dependent bioluminescence when applying coelenterazine systemically through lateral ventricle and a calcium agonist directly in cortex (2/2)

Description:
This is a movie showing Calcium Dependent Bioluminescence in a mouse expressing LMC4 in left primary somatosensory cortex. Water soluble Coelenterazine (CTZ) was injected in …
Year:
2017
Contributor:
Gomez-Ramirez, Manuel (Creator)
Subject:
bioluminescent optogenetics
BL-OG
Bioluminescence
coelenterazine
somatosensory cortex
LMC4
lumicampsin
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Multi-site Multi-modality Imaging Methods for Studying Spinal, Brain, and Vascular Dynamics

Description:
Using neuroscientists' tools of the trade one can image in independent animals either the brain or the spinal cord. Yet understanding many biological processes such …
Year:
2022
Contributor:
Celinskis, Dmitrijs (Creator)
Black, Chris Black, (Creator)
Murphy, Jeremy (Creator)
Friedman, Nina (Creator)
Shaner, Nathan (Creator)
Hochgeschwender, Ute (Creator)
Lipscombe, Diane (Creator)
Saab, Carl (Creator)
Moore, Christopher I. (Creator)
Borton, David A. (Creator)
Subject:
Neuroscience
Bioluminescence
microscopy
imaging
fluorescence
Brain
spinal cord
miniscope
sensory perception
surgical techniques
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Next generation tools for imaging in the cortex and spinal cord

Description:
State-of-the-art imaging tools rely on a restrained preparation, limiting natural behavior while measuring cellular activity via fluorescence (FL). Recently, advances in wearable microscopy expanded the …
Year:
2021
Contributor:
Celinskis, Dmitrijs (Author)
Murphy, Jeremy (Author)
Shaner, Nathan (Author)
Hochgeschwender, Ute (Author)
Lipscombe, Diane (Author)
Moore, Christopher I. (Author)
Borton, David (Author)
Subject:
Bioluminescence
microscopy
imaging
fluorescence
cortex spinal cord
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Non-invasive optogenetic stimulation in a rat model of spinal cord injury

Description:
Despite ever improving treatment methods, there still is no way to effectively restore function after spinal cord injury. A promising approach is to re-engage spinal …
Year:
2019
Contributor:
Kendziorski, Griffin (Creator)
Petersen, Eric (Creator)
Shafau, Lateef (Creator)
Prakash, Mansi (Creator)
Hochgeschwender, Ute (Creator)
Subject:
BL-OG
Bioluminescence
coelenterazine
Optogenetics
spinal cord injury
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Novel Luciferase – Opsin Combinations for Improved Bioluminescent Optogenetics

Description:
Bioluminescent Optogenetics (BL-OG) is a novel approach for controlling neuronal activity in which light-sensing opsins are controlled by “biological light.” Luciferases, enzymes that produce bioluminescent …
Year:
2022
Contributor:
Gorantla, Nipun (Author)
Simkins, Jacob (Author)
Tree, Maya (Author)
Riselay, Kathleen (Author)
Ikefuama, Ebenezer (Author)
Crespo, Emmanuel (Author)
Prakash, Mansi (Author)
Hochgeschwender, Ute (Author)
Subject:
Bioluminescence
luminopsin
Optogenetics
opsin
Luciferase
Chemogenetics
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Optimizing Bioluminescence-Induced Photoactivation of Transcription

Description:
To understand how groups of neurons participate in neural circuits underlying behavior, technologies are needed for observing and controlling the ensembles employed during behavior across …
Year:
2022
Contributor:
Slaviero, Ashley (Author)
Simkins, Jacob (Author)
Crespo, Emmanuel L. (Author)
Hochgeschwender, Ute (Author)
Subject:
Bioluminescence
Optogenetics
calcium
Transcription
light sensitivity
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Orientation and Advancement of the ICV Cannula

Description:
The goal of all methods described here are to place the deep-end of the ICV cannula 2mm below the marking of the skull at ML …
Year:
2018
Contributor:
Klein, Eric (Creator)
Subject:
Injections, Intracerebroventricular
Cannula
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Restoring function after severe spinal cord injury through bioluminescence-driven optogenetic stimulation of spinal circuitry

Description:
A non-invasive and highly specific approach to neuronal stimulation is bioluminescent-optogenetics, where genetically expressed light emitting luciferases are tethered to light sensitive channelrhodopsins (luminopsins, LMO); …
Year:
2018
Contributor:
Petersen, Eric D. (Creator)
Shafau, Lateef (Creator)
Pal, Akash (Creator)
Zenchak, Jessica (Creator)
Pena, Alex (Creator)
Prakash, Mansi (Creator)
Hochgeschwender, Ute (Creator)
Subject:
Bioluminescence
coelenterazine
LMO3
luminopsin
CTZ
spinal cord injury
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Selective Behavioral and Circuit Deficits in Adulthood Following Widespread Postnatal Excitation of Neocortical Pyramidal Neurons

Description:
The amount and pattern of neuronal activity present during the early developmental period is believed to guide structural and functional properties of neural circuit assembly. …
Year:
2019
Contributor:
Björefeldt, Andreas (Creator)
Medendorp, William E. (Creator)
Pal, Akash (Creator)
Waddell, Madison L. (Creator)
Moore, Christopher I. (Creator)
Hochgeschwender, Ute (Creator)
Subject:
BL-OG
Bioluminescence
LMO3
Optogenetics
development
pyramidal neurons
models of autism
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Selective Behavioral and Circuit Deficits in Adulthood Following Widespread Postnatal Excitation of Neocortical Pyramidal Neurons

Description:
The amount and pattern of neuronal activity present during the early developmental period is believed to guide structural and functional properties of neural circuit assembly. …
Year:
2019
Contributor:
Björefeldt, Andreas (Creator)
Medendorp, William E. (Creator)
Pal, Akash (Creator)
Waddell, Madison L. (Creator)
Moore, Christopher I. (Creator)
Hochgeschwender, Ute (Creator)
Subject:
BL-OG
Bioluminescence
LMO3
Optogenetics
development
pyramidal neurons
models of autism
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Selective control of synaptically-connected circuit elements by interluminescence

Description:
A wealth of new tools can directly control output of specific neurons on fast (e.g., optogenetic) or sustained (e.g., chemogenetic) time scales. In contrast, almost …
Year:
2021
Contributor:
Hochgeschwender, Ute (Author)
Shaner, Nathan C. (Author)
Moore, Christopher I. (Author)
Subject:
Bioluminescence
Optogenetics
interluminescence
Chemogenetics
synapse
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Submersible Checklist

Description:
This is a supplementary resource for the Bioluminescence Hub's Bioluminescent Dive, Part 1: Submersibles activity.
Year:
2020
Contributor:
Literman, Krystal (Author)
Subject:
oceanography
education
outreach
STEM practices
Submersibles
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Educational and Engagement Materials
Open Educational Resources (OERs) and Advancing Research Impacts
Full Record

Targeted Circuit Manipulation for Ameliorating Huntington's Disease Pathogenesis

Description:
Almost 30 years after identifying the genetic mutation underlying Huntington’s disease (HD), treatments remain limited to managing late-stage symptoms of motoric, psychiatric, and cognitive deficits. …
Year:
2023
Contributor:
Ikefuama, Ebenezer C. (Creator)
Schalau, R.C. (Creator)
Uprety, Anusha M. (Creator)
Tree, Maya O. (Creator)
Dunbar, G. L. (Creator)
Rossignol, J. (Creator)
Hochgeschwender, Ute (Creator)
Subject:
Bioluminescence
Optogenetics
pyramidal neurons
Huntington's Disease
circuit manipulation
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Techniques for optimal in vivo imaging of genetically engineered bioluminescent indicators and actuators

Description:
Bioluminescent molecules for imaging and modifying neural activity are increasingly becoming viable tools for in vivo use and have the potential to overcome many of …
Year:
2019
Contributor:
Murphy, Jeremy W. (Creator)
Friedman, Nina (Creator)
Klein, Eric (Creator)
Marsh, Sophie (Creator)
Lipscombe, Diane (Creator)
Shaner, Nathan (Creator)
Hochgeschwender, Ute (Creator)
Gomez-Ramirez, Manuel (Creator)
Moore, Christopher I. (Creator)
Subject:
bioluminescent optogenetics
Bioluminescence
imaging
in vivo
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Testing for “off-target” neurophysiological effects of coelenterazine

Description:
Coelenterazine (CTZ) is the substrate of many luciferases and photoproteins critical for native and applied bioluminescence. CTZ has a long track-record and is welltolerated across …
Year:
2019
Contributor:
Cruikshank, Scott J. (Author)
Prakash, Mansi (Author)
Gomez-Ramirez, Manuel (Author)
St. Laurent, Robyn (Author)
More, Alexander I. (Author)
Medendorp, William E. (Author)
Kauer, Julie A. (Author)
Hochgeschwender, Ute (Author)
Connors, Barry W. (Author)
Moore, Christopher I. (Author)
Subject:
Bioluminescence
coelenterazine
Optogenetics
CTZ
Neural effects
HCN
luciferin
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Tracking neocortical dynamics using genetically-encoded bioluminescent molecules in vivo

Description:
As an alternative to fluorescent imaging, we are developing calcium-dependent, genetically-encoded molecules using bioluminescent probes. Bioluminescence is chemically generated light that occurs when a photon-containing …
Year:
2018
Contributor:
Gomez-Ramirez, Manuel (Creator)
More, Alexander I. (Creator)
Friedman, Nina G. (Creator)
Murphy, Jeremy M. (Creator)
Pal, Akash (Creator)
Lipscombe, Diane (Creator)
Hochgeschwender, Ute (Creator)
Moore, Christopher I. (Creator)
Subject:
Bioluminescence
LMC4
lumicampsin
calcium indicator
imaging
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Trans-synaptic Neuronal Communication via Bioluminescent Optogenetics

Description:
Bioluminescent optogenetics (BL-OG) can be utilized for trans-synaptic neuronal communication by expressing the light emitting luciferase in the presynaptic and the light sensing opsin in …
Year:
2020
Contributor:
Schumaker, Rachel (Author)
Prakash, Mansi (Author)
Crespo, Emmanuel (Author)
Hochgeschwender, Ute (Author)
Subject:
BL-OG
Bioluminescence
Optogenetics
synaptic communication
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Full Record

Transsynaptic Neuronal Activation via Bioluminescent Optogenetics

Description:
Biological light activation of optogenetic sensors across synaptic partners offers the potential to optogenetically dissect synaptic communication non-invasively. The genetically encoded light source, a luciferase, …
Year:
2019
Contributor:
Schumaker, Rachel (Creator)
Prakash, Mansi (Creator)
Pal, Akash (Creator)
Crespo, Emmanuel (Creator)
Hochgeschwender, Ute (Creator)
Subject:
BL-OG
Bioluminescence
coelenterazine
Optogenetics
synaptic communication
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Data and Research Products
Available to Brown-affiliated users only
Full Record

Use of Lamprey as a Model for Studying the Neural Control of Locomotion

Description:
This laboratory exercise uses the sea lamprey (Petromyzon marinus) as a model system to examine expression of genetically-encoded neural activity probes (LMO3 and GCaMP6) and …
Year:
2019
Contributor:
Morgan, Jennifer (Creator)
Subject:
Bioluminescence
LMO3
spinal cord
Lamprey
Collection:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging
NeuroNex Educational and Engagement Materials
Full Record

Items (61-80) out of 87 results