The NeuroNex Technology Hub and NeuroNex Bioluminescent Constructs Database are supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award …
The NeuroNex Technology Hub and NeuroNex Bioluminescent Constructs Database are supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award …
BioLuminescent-OptoGenetic molecules for driving and imaging neural activity have a number of advantages in vivo. In this approach, genetically expressed luciferases bind with injected luciferins …
We developed a bioluminescent Ca2+ indicator, Lumicampsin (LMC) that employs a split, mutated Gaussia luciferase (sbGluc) with a CaM-M13 calcium sensing moiety introduced between the …
Transgenic neuromodulation tools (i.e., optogenetics and chemogenetics) enable the manipulation of brain activity using targeted genetic delivery strategies for transducing specific cells to produce transgenic …
Animal behavior is driven by perception of biological need and environmental context. Internal representations of the external world help us to interact with and navigate …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
The Bioluminescence Hub, a National Science Foundation NeuroNex Technology Hub, is dedicated both to the development of novel bioluminescent and optogenetic tools and to the …
Fluorescence-based optogenetic tools are powerful methods for dissecting cell-type specific circuit dynamics in the nervous system. These tools, however, rely on external, generic modification of …
Chemogenetic modulation of neuronal populations allows for precise control over cells engineered to express an exogenous receptor that responds exclusively to specific molecules. Chemogenetic approaches …
Though critical for life, the sensation of pain is also a debilitating symptom of many disorders. While understanding its transduction may provide valuable insight into …
In this thesis work, we have presented the concept and fabrication of the single coaxial optrode. Its dual functionality of simultaneous light delivery and electrophysiological …
The perirhinal cortex (PER) has a well-established role in identification and recognition of objects. Our lab previously provided evidence that neuronal activity in PER may …
Using optogenetics, we can manipulate neuronal activity using light. Due to the young age of the animals used in this study, a non-invasive light source …
Neuroprosthesis research aims to enable communication between the brain and external assistive devices while restoring lost functionality such as occurs from stroke, spinal cord injury …
The malformation of neuronal circuitry in the developing neocortex leads to a variety of psychiatric disorders later in the organism’s life. By investigating alterations in …
This digital poster created for the 2021 BRAIN Initiative Investigators Meeting provides an overview of the advantages of bioluminescence for neuroscience research. Our BioLuminescent-OptoGenetics (BL-OG) …
Epilepsy is a common neurological disorder characterized by the recurrence of spontaneous seizures. However, the mechanisms of transition into seizures are still unclear. A better …
Patterns of neuronal activity during early development are believed to guide the assembly of neural circuits. Thus, alterations of neuronal activity in this time window …
