In this thesis work, we have presented the concept and fabrication of the single coaxial optrode. Its dual functionality of simultaneous light delivery and electrophysiological recording has been demonstrated. We applied the coaxial optrode as a stand-alone unit in in vivo optical stimulation and recording in various experimental animal models - including transgenic mice, optogenetically transduced rodents and non-human primates. To better understand light distribution and compare optical stimulation among different fiber optical structures, we investigated the interaction between light and brain tissue, and then employed the Monte Carlo method to simulate the process of light propagation and associated issue of heating. As suggested by the simulation result, we expect relatively mild temperature increases in response to stimulation paradigms currently used in our lab. We also construct a model that describes the electric behavior of optrode, which allows the spatial sensitivity of optrodes with various coating configurations to be mathematically analyzed.<br/> In the second part of this thesis, we demonstrate an integrated optrode-microelectrode array (optrode-MEA) device that enables neuromodulation and simultaneous recording of electrophysiological responses of neuronal populations in ChR2-expressing rodents in vivo. We have showed the use of this device as an optogenetic tool for single site optical stimulation and electrical mapping of neural activity in a configuration which can be applied to other animal models. Finally, to translate these optical neuromodulation studies into non-human primates, we are working on the polymer fiber-MEA version of the device that allows multisite optical stimulation and electrical recording. Such a device could be desirable for potential clinical trials in human patients.
Wang, Jing,
"COMBINED OPTICAL STIMULATION AND ELECTRICAL RECORDING IN IN-VIVO NEUROMODULATION"
(2012).
Physics Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://doi.org/10.7301/Z0KP80GJ
