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Year:: 2022
Contributor:: Gorantla, Nipun (Author); Simkins, Jacob (Author); Tree, Maya (Author); Riselay, Kathleen (Author); Ikefuama, Ebenezer (Author); Crespo, Emmanuel (Author); Prakash, Mansi (Author); Hochgeschwender, Ute (Author)
Subject:: Bioluminescence; luminopsin; Optogenetics; opsin; Luciferase; Chemogenetics
DOI: https://doi.org/10.26300/5czy-xh02

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Abstract:: Bioluminescent Optogenetics (BL-OG) is a novel approach for controlling neuronal activity in which light-sensing opsins are controlled by “biological light.” Luciferases, enzymes that produce bioluminescent light by oxidizing chemicals known as luciferins, are tethered to opsins to create a luminopsin construct with the capability to utilize bioluminescent light for neuronal excitation or inhibition. To maximize the generation of BL-OG induced neuronal activity, variants of luminopsin constructs containing various combinations of light-emitters and light sensors were developed. Novel constructs included super-sensitive channel rhodopsins, blue- and red-light sensors, and light-sensing G-Protein coupled receptors. This project aims to examine the relative efficiency of various constructs by evaluating them through Multielectrode Array Recordings of primary neuronal cultures by gauging their effects on neuronal activity following stimulation with physical and biological light sources.
Notes:: This research was supported by the the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352

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Use and Reproduction: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Rights: In Copyright

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Gorantla, Nipun, Simkins, Jacob, Tree, Maya, et al., "Novel Luciferase – Opsin Combinations for Improved Bioluminescent Optogenetics" (2022). NeuroNex Data and Research Products. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/5czy-xh02

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NeuroNex Data and Research Products

This collection contains publicly available research products, including data, codes, and publications, among others. The NeuroNex Technology Hub is supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352.
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Novel Luciferase – Opsin Combinations for Improved Bioluminescent Optogenetics