Description
- Abstract:
- Extracellular vesicles (EVs) have emerged as a key biomarker in liquid biopsy diagnostics, due to their abundance in bodily fluids and their ability to reflect the physiological state of their cells of origin. Their diverse functions, including immune regulation to intercellular communication, underscore their potential in biomedical research. This study focused on two main objectives: (1) optimization of digital ELISA assays for the detection of breast cancer-derived EVs, and (2) improvement of EV extraction methods from dried blood spots (DBS). The Single Molecule Array technology was used for the detection of both EVs. In the first study, breast cancer samples showed an increase in CD63 expression compared to normal controls. In the second study, there is evidence of faster EV extraction (from 1 hr. to 5 minutes) after applying an electric field to the dried blood spot compared to existing methods in literature.
- Notes:
- Thesis (Sc. M.)--Brown University, 2025
Citation
Lujuo, Christine,
"Exploring the Diagnostic Potential of Extracellular Vesicles"
(2025).
Biomedical Engineering Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://repository.library.brown.edu/studio/item/bdr:44n7a688/
Relations
Collection:
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Biomedical Engineering Theses and Dissertations
Theses and Dissertations for the Biomedical Engineering department....