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A CRISPR-based strategy for targeted sequencing in biodiversity science

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Overview

Year:
2023
Contributor:
Littleford-Colquhoun, Bethan (Creator)
Kartzinel, Tyler (Creator)
Subject:
metagenomics
amplicon sequencing
barcoding
Cas9
environmental DNA
guide RNA
metabarcoding
long-read sequencing
contigs
Flye
DOI
https://doi.org/10.26300/m1kr-3q85

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Description

Abstract:
The bioinformatic steps taken for experiments 1-6 in "A CRISPR-based strategy for targeted sequencing in biodiversity science" by Littleford-Colquhoun & Kartzinel 2023. These bioinformatic steps taken for each experiment include: 1) Adapter trimming quality (Q>=8) Nanopore sequence reads using Porechop 2) Error correcting trimmed reads using Canu 3) Assembling contigs using Flye

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Access Conditions

Use and Reproduction
CC0 1.0 Universal (CC0 1.0)
Rights
No Copyright - United States

Citation

Littleford-Colquhoun, Bethan, and Kartzinel, Tyler, "A CRISPR-based strategy for targeted sequencing in biodiversity science" (2023). Brown University Open Data Collection. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/m1kr-3q85

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Collection:

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    Brown University Open Data Collection

    This collection contains open and public access data sets created by Brown University faculty and student researchers. Increasingly, publishers and funders are requiring that protocols, data sets, documentation and code underlying publications be retained and preserved in repositories, their locations …
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