Testing Nebivolol&#x27;s Effects on Zebrafish Swim Patterns Using a Behavioral Assay

Brown, Cameron

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Year:: 2022
Contributor:: Brown, Cameron (creator); Creton, Robbert (Advisor); Kreiling, Jill (Reader); Brown University. Biology and Medicine: Pathobiology (sponsor)
Genre:: theses
Subject:: Cyclosporine--Therapeutic use; Alzheimer's disease; Zebrafish Behavior
Extent:: 6, 38 p.

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Abstract:: Alzheimer’s is a debilitating neurodegenerative disease that results in memory loss, difficulty with language and learning, and overall impairments in everyday life. Currently, there is not an FDA-approved drug that can fully treat and prevent the progression of Alzheimer’s. A potential therapeutic option is the inhibition of a protein phosphatase expressed in the brain called calcineurin. When excessively produced, calcineurin can further contribute to the progression of Alzheimer’s. A popular calcineurin inhibitor, and a drug of interest to treat Alzheimer’s, is an immunosuppressant called Cyclosporine A. While cyclosporine has shown neuroprotective effects, its side effects have made it difficult to be used as a treatment for Alzheimer’s. Researchers now set out to find a compound similar to cyclosporine that is also tolerable and safe, and Nebivolol Hydrochloride falls into that category. Nebivolol, typically used for hypertension, has shown that it can elicit neuroprotection and similar behavioral effects in zebrafish as cyclosporine. In this study, confirmation and concentration experiments were carried out to confirm if nebivolol does behave similarly to cyclosporine and to see what concentrations of the compound provide effects on zebrafish behavior most similarly to cyclosporine. Using a behavioral assay, this study was able to determine that nebivolol does have the same effect on zebrafish behavior as cyclosporine and it provided new information on how different concentrations of the compound affect the fish. After the experiments, swim pattern analysis was used to visualize how the larval movements were affected by nebivolol during the behavioral assay.
Notes:: Thesis (Sc. M.)--Brown University, 2022

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Citation

Brown, Cameron, "Testing Nebivolol's Effects on Zebrafish Swim Patterns Using a Behavioral Assay" (2022). Pathobiology Theses and Dissertations. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:5hww2ztq/

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Pathobiology Theses and Dissertations

Theses and Dissertations for the Pathobiology department.
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Testing Nebivolol's Effects on Zebrafish Swim Patterns Using a Behavioral Assay