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In vitro and histological analysis of polymer microsphere delivery


Cellular recruitment has diverse tissue engineering applications, ranging from wound healing to the treatment of metabolic disorders. Chemoattractants encapsulated in hydrogels, microspheres, scaffolds, and other systems have been studied as recruitment agents for a variety of these applications. Thus the goal of our project is to study how microspheres can be used to recruit progenitor cells. To achieve this, we histologically analyzed the presence of non-degradable polystyrene microspheres in rats delivered via either oral gavage or subcutaenous injection. These spheres serve as a model for bioresorbable polylactic acid microspheres. Next, we examined the effects that various solvents used during the histology process have on polymers, as their use can cause artefacts in histology slides. Lastly, we assayed cell response to dexamethsone, a drug involved in adipogenic differention. This work furthers our understanding of cell recruitment through microspheres and may aid in further development of recruitment based therapies


Yakubov, Jonathan, Zhou, George, Léandre, Vérida, et al., "In vitro and histological analysis of polymer microsphere delivery" (2016). Summer Research Symposium. Brown Digital Repository. Brown University Library.



  • Summer Research Symposium

    Each year, Brown University showcases the research of its undergraduates at the Summer Research Symposium. More than half of the student-researchers are UTRA recipients, while others receive funding from a variety of Brown-administered and national programs and fellowships and go …