Description
- Abstract:
- This project aimed (1) to learn the fundamental mechanism of DNA extraction on a novel microfluidic chip that takes advantage of hydrophilic/hydrophobic interactions between sample solutions and channel liquids and (2) to evaluate the feasibility of applying microfluidic chip technology to non-invasive prenatal diagnostics. A simple and efficient method of extracting cell-free fetal DNA circulating in maternal blood serum is necessary to expand applications of non-invasive prenatal diagnostics in clinical settings; a microfluidic chip is a suitable device for point-of-care DNA extraction. In this project, 200 base pair (bp) DNA was used to substitute for cell-free fetal DNA that is characterized by its short, fragmented nature. Three on-chip experiments were conducted: (1) extraction of DNA diluted in TE buffer (10 mM Tris-HCL, 0.1 mN EDTA), (2) extraction of DNA diluted in TE buffer with the addition of carrier beads, and (3) extraction of DNA diluted in human blood serum. With successful recovery of 200 bp DNA at 12.5 – 57.6 % yield in all three experiments, we can ascertain that our microfluidic chip system is suitable for extraction of short-fragmented DNA in TE and human blood serum. When accompanied by advances in microfluidic chip fabrication and DNA extraction, our system could serve as a method of efficient and accurate point-of-care non-invasive prenatal diagnostic.
- Notes:
- Thesis (Sc. M.)--Brown University, 2017
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Citation
Lee, Ga Yeon,
"Extracting short-fragmented DNA from human blood serum samples using a novel microfluidic chip device"
(2017).
Biomedical Engineering Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://doi.org/10.7301/Z01J9869
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Biomedical Engineering Theses and Dissertations
Theses and Dissertations for the Biomedical Engineering department....