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Year:: 2014
Contributor:: Catlina, Natasha R. (Author); Husea, Susan M. (Author); Boekelheide, Kim (Author)
Subject:: laser capture microdissection; LCM; testicular germ cell; apoptotic response; PCR array; qRT-PCR array

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Abstract:: This is a post-print of the article allowed by the publisher to be deposited in the Brown Digital Repository. We optimized the LCM method optimized to generate RNA quantities sufficient for qRT-PCR array analysis without amplification, and were able to validate the method through direct comparison of results from unamplified and amplified RNA from individual samples. The addition of an amplification step for gene expression studies using LCM RNA resulted in a bias, especially for low abundance transcripts. Although the amplification bias was consistent across samples, researchers should use caution when comparing results generated from amplified and unamplified LCM RNA.
Notes:: This work received funding from the National Institute of Environmental Health Sciences grant P42ES013660Â

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Catlina, Natasha R., Husea, Susan M., and Boekelheide, Kim, "Author manuscript: 'The stage-specific testicular germ cell apoptotic response to low-dose X-irradiation and 2,5-hexanedione combined exposure. I: Validation of the laser capture microdissection method for qRT-PCR array application'" (2014). Brown Superfund Project 1: Molecular Biomarkers for Assessing Testicular Toxicity Digital Archive, Brown Superfund Presentations & Publications. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:792926/

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Brown Superfund Project 1: Molecular Biomarkers for Assessing Testicular Toxicity Digital Archive

This archive is for the long-term preservation and public dissemination of data and digital research products collected and created by researchers associated with Brown University Superfund Research Program Project 1: "Molecular Biomarkers for Assessing Testicular Toxicity". Project Leader: Kim Boekelheide, …
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Brown Superfund Presentations & Publications

This collection contains research articles, conference papers, research posters, and slide presentations associated with Brown University Superfund Research Program's investigators and research projects.
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Author manuscript: "The stage-specific testicular germ cell apoptotic response to low-dose X-irradiation and 2,5-hexanedione combined exposure. I: Validation of the laser capture microdissection method for qRT-PCR array application"