Description
- Abstract:
- Organism: Rattus norvegicus and Experiment type: Expression profiling by array. Summary: Analysis of gene expression and alternate splicing effects of retinoic acid treatment on gestational day 15 rat fetal testes in whole testis culture. Retinoic acid exposure in cultured fetal testis has previously been demonstrated to have significant effects on the histology of the fetal testis in multiple species, as well as to alter the meiotic states of germ cells. However, previous experiments have not analyzed the mechanisms by which retinoic acid exposure leads to altered tubulogenesis and loss of seminiferous cord structure. This experiment demonstrated that retinoic acid exposure activated signaling pathways that promote the ovary development program and oppose normal testis development in mid-gestational rat fetal testes. Overall design: Testes were isolated from rats on gestational day 15. A paired design was used in which a single testis from each of six rat fetuses was cultured in 10^-6 M all-trans retinoic acid (ATRA), while the contralateral testis was cultured in vehicle (DMSO) media. After 24 h culture, total RNA was isolated, and gene expression and alternate splicing were analyzed using the Affymetrix Clariom D Rat assay.
- Notes:
- This research is supported by the National Institute of Environmental Health Sciences (NIEHS) Superfund Research Program P42ES013660
Access Conditions
- Use and Reproduction
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- Attribution 4.0 International (CC BY 4.0)
Citation
Spade, DJ, Schorl, C., Dere, E., et al.,
"Short-term in vitro culture of rat fetal testes with all-trans retinoic acid"
(2019).
Brown Superfund Data Products, Brown Superfund Project 1: Molecular Biomarkers for Assessing Testicular Toxicity Digital Archive, Brown University Open Data Collection.
Brown Digital Repository. Brown University Library.
https://doi.org/10.26300/kx5e-y244
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Brown Superfund Data Products
This collection contains data sets, code, and documentation files associated with the Brown University Superfund Research Program's investigators and research projects.... -
Brown Superfund Project 1: Molecular Biomarkers for Assessing Testicular Toxicity Digital Archive
This archive is for the long-term preservation and public dissemination of data and digital research products collected and created by researchers associated with Brown University Superfund Research Program Project 1: "Molecular Biomarkers for Assessing Testicular Toxicity". Project Leader: Kim Boekelheide, …... -
Brown University Open Data Collection
This collection contains open and public access data sets created by Brown University faculty and student researchers. Increasingly, publishers and funders are requiring that protocols, data sets, documentation and code underlying publications be retained and preserved in repositories, their locations …...