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Year:: 2022
Contributor:: Ikefuama, Ebenezer (Author); Kendziorski, Griffin (Author); Anderson, Kevin (Author); Riselay, Kathleen (Author); Tree, Maya (Author); Prakash, Mansi (Author); Hochgeschwender, Ute (Author); Petersen, Eric D. (Author)
Subject:: Bioluminescence; Optogenetics; spinal cord injury; Luciferase
DOI: https://doi.org/10.26300/52w5-ry49

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Abstract:: Despite ever improving treatment methods, there still is no way to effectively restore function after spinal cord injury. A promising approach is to re-engage spinal cord neurons caudal to the injury site. A potential method to achieve this is optogenetics. Traditionally, optogenetic stimulation allows activation of neurons using an external light source. However, the invasiveness and need for an implanted optical fiber severely limits the viability of this strategy for treating spinal cord injuries. In this study, a light-producing luciferase (sbGLuc, a variant of Gaussia luciferase) was used as an internal light source fused to a highly light-sensitive blue-shifted channelrhodopsin, CheRiff, to generate a luminescent opsin (luminopsin, LMO). Cells expressing the LMO are activated through bioluminescence following an intraperitoneal injection of the luciferin coelenterazine (CTZ). When activated, the light sensitive opsin opens, and the flow of cations results in the excitation of the neuron (BioLuminescent OptoGenetics, BL-OG). Previous work from this lab has found that BL-OG activation of neurons caudal to the lesion site improved locomotor function. By employing an opsin with increase light sensitivity, the current work specifically aims to analyze the efficacy of intraperitoneal injection of CTZ as opposed to the more invasive lateral ventricle cannula method.
Notes:: This research was supported by the the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352 and CBET-1464686 and the Craig H. Neilsen Foundation

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Use and Reproduction: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Rights: In Copyright

Citation

Ikefuama, Ebenezer, Kendziorski, Griffin, Anderson, Kevin, et al., "Improved Locomotor Recovery in a Rat Model of Spinal Cord Injury by Non-Invasive BioLuminescent-Optogenetic (BL-OG) Stimulation" (2022). NeuroNex Data and Research Products. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/52w5-ry49

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NeuroNex Data and Research Products

This collection contains publicly available research products, including data, codes, and publications, among others. The NeuroNex Technology Hub is supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352.
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Improved Locomotor Recovery in a Rat Model of Spinal Cord Injury by Non-Invasive BioLuminescent-Optogenetic (BL-OG) Stimulation