Description
- Abstract:
- Pre-vascularization of engineered tissue constructs by making patterned vessels is a growing need in the tissue engineering field as the research moves towards making larger constructs for clinical applications. The patterned vessel network helps with providing oxygen and nutrients necessary for survival of the cells within the construct and also helps with better engraftment of the construct with the host tissue. A common way of making the patterned vessels is 3D printing of a sacrificial material and seeding the pattern with endothelial cells after the sacrificial material is removed. The purpose of this work is to optimize two hydrogels for core-shell bioprinting for the patterned vessels where the core would be a sacrificial hydrogel, and the shell would be a hydrogel containing endothelial cells, thus removing the process of seeding the patterned vessels later with endothelial cells. The two hydrogels are optimized separately by printing them through a single- needle extrusion bioprinter. 3% (w/v) alginate is finalized as the core hydrogel by bioprinting different concentrations of alginate and comparing the width of the printed filaments and characterizing the changes that occur by changing the printing parameters. A combination of 10% (w/v) gelatin, 0.5% (w/v) alginate and 1% (w/v) fibrinogen is finalized for the shell hydrogel that will contain the cells by bioprinting different concentrations of gelatin, alginate and fibrinogen. The cytotoxicity of alginate was tested against endothelial cells as the cells will come in contact with the alginate when printed into a core-shell fashion. Finally, cells were printed by mixing them with the gelatin/alginate/fibrinogen hydrogel and stained using a Live/Dead stain to see if the cells can be printed with the gelatin/alginate/fibrinogen hydrogel. The presence of live cells confirmed that the combination of gelatin/alginate/fibrinogen can be used for printing endothelial cells.
- Notes:
- Thesis (Sc. M.)--Brown University, 2023
Citation
Singh, Yamini,
"Optimization of Hydrogels for Core-Shell 3D Bioprinting of Patterned Vessels for Vascularization Engineered Cardiac Tissues"
(2023).
Biomedical Engineering Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://repository.library.brown.edu/studio/item/bdr:kmjruhuc/
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Biomedical Engineering Theses and Dissertations
Theses and Dissertations for the Biomedical Engineering department....