We developed a bioluminescent Ca2+ indicator, Lumicampsin (LMC) that employs a split, mutated Gaussia luciferase (sbGluc) with a CaM-M13 calcium sensing moiety introduced between the …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
Bioluminescent sensors of intracellular calcium offer a number of advantages in vitro and in vivo. Calcium-sensing luciferases can be further tethered to specific voltage-gated Ca …
The Bioluminescence Hub, a National Science Foundation NeuroNex Technology Hub, is dedicated both to the development of novel bioluminescent and optogenetic tools and to the …
Fluorescence-based optogenetic tools are powerful methods for dissecting cell-type specific circuit dynamics in the nervous system. These tools, however, rely on external, generic modification of …
In BioLuminescent OptoGenetics (BL-OG), the light sensing opsin, such as a channelrhodopsin or a pump, is activated by biological light emitted from a luciferase that …
Using neuroscientists' tools of the trade one can image in independent animals either the brain or the spinal cord. Yet understanding many biological processes such …
Bioluminescent molecules for imaging and modifying neural activity are increasingly becoming viable tools for in vivo use and have the potential to overcome many of …
As an alternative to fluorescent imaging, we are developing calcium-dependent, genetically-encoded molecules using bioluminescent probes. Bioluminescence is chemically generated light that occurs when a photon-containing …