BioLuminescent-OptoGenetic molecules for driving and imaging neural activity have a number of advantages in vivo. In this approach, genetically expressed luciferases bind with injected luciferins …
As bioluminescence becomes increasingly significant to imaging and manipulating neurons in the brain, there presents a need for a database that can concisely present bioluminescent …
We developed a bioluminescent Ca2+ indicator, Lumicampsin (LMC) that employs a split, mutated Gaussia luciferase (sbGluc) with a CaM-M13 calcium sensing moiety introduced between the …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
Bioluminescence imaged using BLmini v2.0 in an anesthetized wild-type animal with the cortical application of luciferin (hCTZ). Bioluminescent calcium indicator was expressed via intracortical injection …
Bioluminescence imaged using BLmini v2.0 in an anesthetized wild-type animal with the cortical application of luciferin (hCTZ). Bioluminescent calcium indicator was expressed via intracortical injection …
Bioluminescent sensors of intracellular calcium offer a number of advantages in vitro and in vivo. Calcium-sensing luciferases can be further tethered to specific voltage-gated Ca …
In vivo fluorescence miniature microscopy has recently proven a major advance, enabling cellular imaging in freely behaving animals. However, fluorescence imaging suffers from autofluorescence, phototoxicity, …
Categories serve to reduce complexity and help us to navigate our social environment. At the same time, they can trap individuals in perceived or real …
The Bioluminescence Hub, a National Science Foundation NeuroNex Technology Hub, is dedicated both to the development of novel bioluminescent and optogenetic tools and to the …
Fluorescence-based optogenetic tools are powerful methods for dissecting cell-type specific circuit dynamics in the nervous system. These tools, however, rely on external, generic modification of …
Chemogenetic modulation of neuronal populations allows for precise control over cells engineered to express an exogenous receptor that responds exclusively to specific molecules. Chemogenetic approaches …
Interluminescence is a method for selective modulation of synaptic communication between genetically defined partners. Bioluminescent light from a pre-synaptic released luciferase activates a post-synaptic localized …
Using optogenetics, we can manipulate neuronal activity using light. Due to the young age of the animals used in this study, a non-invasive light source …
The malformation of neuronal circuitry in the developing neocortex leads to a variety of psychiatric disorders later in the organism’s life. By investigating alterations in …
This digital poster created for the 2021 BRAIN Initiative Investigators Meeting provides an overview of the advantages of bioluminescence for neuroscience research. Our BioLuminescent-OptoGenetics (BL-OG) …
Patterns of neuronal activity during early development are believed to guide the assembly of neural circuits. Thus, alterations of neuronal activity in this time window …
