The RspE gene is responsible for encoding a portion of the 30S ribosomal subunit in B. subtilis. Single nucleotide mutations in the RspE gene provide protection from the effects of the ribosome targeting antibiotic spectinomycin. The goal of the project is to study the impacts of genotoxic agents on the mutations in the RspE gene. We will define whether specific treatments result in different mutation rates or a preference for specific nucleotide changes. Sequencing a library of these mutants using the Illumina next generation sequencing platform will allow to characterize the mutations and observe how various genotoxic agents impact mutation types and rates.
"Impacts of genotoxic agents on rates of ribosomal mutations in the 30S ribosomal rspE gene."
Summer Research Symposium.
Brown Digital Repository. Brown University Library.
Each year, Brown University showcases the research of its undergraduates at the Summer Research Symposium. More than half of the student-researchers are UTRA recipients, while others receive funding from a variety of Brown-administered and national programs and fellowships and go …