Description
- Abstract:
- Subcellular mRNA localization is a conserved mechanism across eukaryotes, which is found in both somatic and germ cells. RNA localization in combination with local translation provides a means to generate polarized protein synthesis. In Xenopus laevis oocytes, vg1 mRNA, which encodes a TGFβ growth factor family member, is localized to the vegetal cortex of developing oocytes. Vegetal RNA localization ensures that only the vegetal most blastomeres inherit vg1 mRNA, resulting in proper germ layer patterning. Vg1 mRNA is actively transported as a ribonucleoprotein (RNP) complex, but only a handful of vg1 RNP components are as yet known. Understanding the composition of transport RNPs is critical to reveal not only their molecular nature, assembly, and biochemical properties, but also may provide links to cytoplasmic RNPs in other systems. To that end, we have developed a multi-step approach to biochemically isolate endogenous vegetal transport granules. Proteomic analysis of the transport granules revealed both protein constituents found in other known cytoplasmic RNP granules—including stress granules, processing bodies, neuronal granules and germ granules—as well as a set of unique proteins. Preliminary transcriptome analysis further indicates that vegetal transport granules contain heterotypic populations of RNAs, also consistent with some cytoplasmic granule types. Together our results suggest that vegetal transport RNPs represent a new class of cytoplasmic RNP granules.
- Notes:
- Thesis (Ph. D.)--Brown University, 2018
Access Conditions
- Rights
- In Copyright
- Restrictions on Use
- Collection is open for research.
Citation
Jeschonek, Samantha Page,
"Characterization of Vegetal RNP Transport Granules in Xenopus laevis Oocytes."
(2018).
Molecular Biology, Cell Biology, and Biochemistry Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://doi.org/10.26300/m1mg-nk10
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Molecular Biology, Cell Biology, and Biochemistry Theses and Dissertations
Theses and Dissertations for the Molecular Biology, Cell Biology, and Biochemistry department....