Description
- Abstract:
- An automated counting method was developed in ImageJ to quantify MNGs in digital images of hematoxylin-stained rat fetal testis tissue sections. Timed pregnant Sprague Dawley rats were exposed by daily oral gavage from gestation day 17 to 21 with one of eight phthalate test compounds or corn oil vehicle. Both the manual counting method and the automated image analysis method identified di-n-butyl phthalate, butyl benzyl phthalate, dipentyl phthalate, and di-(2-ethylhexyl) phthalate as positive for induction of MNGs. Dimethyl phthalate, diethyl phthalate, the brominated phthalate di-(2-ethylhexyl) tetrabromophthalate, and dioctyl terephthalate were negative. The correlation between automated and manual scoring metrics was high (r = 0.923). Results of MNG analysis were consistent with these compounds’ anti-androgenic activities, which were confirmed in an ex vivo testosterone production assay. In conclusion, we have developed a reliable image analysis method that can be used to facilitate dose-response studies for the reproducible induction of MNGs by in utero phthalate exposure.
- Notes:
- This research is supported by the National Institute of Environmental Health Sciences (NIEHS) Superfund Research Program P42ES013660
Access Conditions
- Use and Reproduction
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- Attribution 4.0 International (CC BY 4.0)
Citation
Spade, Daniel J., Yue Bai, Cathy, Lambright, Christy, et al.,
"Supplementary data from 'Validation of an automated counting procedure for phthalate-induced testicular multinucleated germ cells'"
(2018).
Brown Superfund Data Products, Brown Superfund Project 1: Molecular Biomarkers for Assessing Testicular Toxicity Digital Archive, Brown University Open Data Collection.
Brown Digital Repository. Brown University Library.
https://doi.org/10.26300/hmt5-kx75
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Brown Superfund Data Products
This collection contains data sets, code, and documentation files associated with the Brown University Superfund Research Program's investigators and research projects.... -
Brown Superfund Project 1: Molecular Biomarkers for Assessing Testicular Toxicity Digital Archive
This archive is for the long-term preservation and public dissemination of data and digital research products collected and created by researchers associated with Brown University Superfund Research Program Project 1: "Molecular Biomarkers for Assessing Testicular Toxicity". Project Leader: Kim Boekelheide, …... -
Brown University Open Data Collection
This collection contains open and public access data sets created by Brown University faculty and student researchers. Increasingly, publishers and funders are requiring that protocols, data sets, documentation and code underlying publications be retained and preserved in repositories, their locations …...