Genetic and Biochemical Characterization of Phospholipase Associated Metabolic Dysfunction in Drosophila melanogaster

Williams, Shawn

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Year:: 2021
Contributor:: Williams, Shawn (creator); DeLong, Alison (Reader); Wharton, Kristi (Reader); Tatar, Marc (Reader); Larschan, Erica (Reader); Gibbs, Allen (Reader); Rand, David (Advisor); Brown University. Department of Molecular Biology, Cell Biology and Biochemistry (sponsor)
Genre:: theses
Subject:: Drosophila melanogaster--Genetics; Lipids--Metabolism; Neurodegeneration; Starvation
Extent:: 12, 127 p.

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Abstract:: Resistance to starvation is a classical complex trait, where varying genetic and environmental landscapes can greatly modify an animal’s ability to cope. In this study, we investigate the genetic basis of starvation resistance using a combination of quantitative and classical genetic mapping. Using the Drosophila Genetics Reference Panel (DGRP) as a starting point, we queried the genetic basis of starvation sensitivity in the most sensitive flies. We localize a major effect locus modifying starvation resistance to the phospholipase iPLA2-VIA. We demonstrate this line has altered phospholipid, lyso-phospholipid, fatty acid and sugar metabolism. In addition, our analyses revealed that the relative composition of the fatty acid transport molecule carnitine was reduced and dietary supplementation with carnitine was sufficient to improve resistance Taken together, this study identifies DGRP line_765 as a model of iPLA2-VIA dysfunction and implicates the mis-regulation of sugar metabolism as a consequence of iPLA2-VIA dysfunction, suggesting a link between defective sugar metabolism and neurodegeneration.
Notes:: Thesis (Ph. D.)--Brown University, 2021

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Citation

Williams, Shawn, "Genetic and Biochemical Characterization of Phospholipase Associated Metabolic Dysfunction in Drosophila melanogaster" (2021). Molecular Biology, Cell Biology, and Biochemistry Theses and Dissertations. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:tbh6w4ne/

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Data from thesis "Genetic and Biochemical Characterization of Phospholipase Associated Metabolic Dysfunction in Drosophila melanogaster"
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Molecular Biology, Cell Biology, and Biochemistry Theses and Dissertations

Theses and Dissertations for the Molecular Biology, Cell Biology, and Biochemistry department.
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