This recorded lecture introduces students to the quantification of "brightness," ways to quantify and compare bioluminescent and fluorescent proteins, and the factors that influence Förster …
The NeuroNex Technology Hub and NeuroNex Bioluminescent Constructs Database are supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award …
BioLuminescent-OptoGenetic molecules for driving and imaging neural activity have a number of advantages in vivo. In this approach, genetically expressed luciferases bind with injected luciferins …
As bioluminescence becomes increasingly significant to imaging and manipulating neurons in the brain, there presents a need for a database that can concisely present bioluminescent …
We developed a bioluminescent Ca2+ indicator, Lumicampsin (LMC) that employs a split, mutated Gaussia luciferase (sbGluc) with a CaM-M13 calcium sensing moiety introduced between the …
This double-sided placemat contains activities for students to complete as they learn about the creatures that produce bioluminescence and the differences between bioluminescence, fluorescence, and …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
This recorded lecture introduces undergraduate students to the advantages of bioluminescence as an optical reporter of a chemical reaction and its use with optogenetics (BL-OG) …
Bioluminescent sensors of intracellular calcium offer a number of advantages in vitro and in vivo. Calcium-sensing luciferases can be further tethered to specific voltage-gated Ca …
In vivo fluorescence miniature microscopy has recently proven a major advance, enabling cellular imaging in freely behaving animals. However, fluorescence imaging suffers from autofluorescence, phototoxicity, …
Categories serve to reduce complexity and help us to navigate our social environment. At the same time, they can trap individuals in perceived or real …
The Bioluminescence Hub, a National Science Foundation NeuroNex Technology Hub, is dedicated both to the development of novel bioluminescent and optogenetic tools and to the …
This recorded lecture introduces undergraduate students to calcium as a ubiquitous second messenger and a proxy for cellular activity. Students learn why calcium is unique, …
Fluorescence-based optogenetic tools are powerful methods for dissecting cell-type specific circuit dynamics in the nervous system. These tools, however, rely on external, generic modification of …
Chemogenetic modulation of neuronal populations allows for precise control over cells engineered to express an exogenous receptor that responds exclusively to specific molecules. Chemogenetic approaches …
