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Year:: 2017
Contributor:: Gomez-Ramirez, Manuel (Creator)
Subject:: bioluminescent optogenetics; BL-OG; Bioluminescence; coelenterazine; somatosensory cortex; LMC4; lumicampsin
DOI: https://doi.org/10.7301/Z0348HW8

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Abstract:: This is a movie showing Calcium Dependent Bioluminescence in a mouse expressing LMC4 in left primary somatosensory cortex. Water soluble Coelenterazine (CTZ) was injected in the lateral ventricle of the right hemisphere, while the calcium agonist (NMDA) was injected directly in cortex. The CTZ concentration was 1μg/μl (2.34 mM concentration, volume 2.5 μl). Bioluminescence was maximal when both CTZ and NMDA were present at the same time. The animal was anesthetized.
Notes:: NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging is funded by National Science Foundation Award #1707352. This research received funding from the NIH National Institute of Neurological Disorders and Stroke (NINDS) Award U01NS099709 and funding from the W. M. Keck Foundation (Bioluminescent Optogenetics to Autoregulate Excitable Cells).

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Use and Reproduction: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Citation

Gomez-Ramirez, Manuel, "Movie: Calcium dependent bioluminescence when applying coelenterazine systemically through lateral ventricle and a calcium agonist directly in cortex (2/2)" (2017). NeuroNex Data and Research Products. Brown Digital Repository. Brown University Library. https://doi.org/10.7301/Z0348HW8

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NeuroNex Data and Research Products

This collection contains publicly available research products, including data, codes, and publications, among others. The NeuroNex Technology Hub is supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352.
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Movie: Calcium dependent bioluminescence when applying coelenterazine systemically through lateral ventricle and a calcium agonist directly in cortex (2/2)