Description
- Abstract:
- Microplastic pollution of marine environments is a pressing problem for the health of aquatic ecosystems and for global food supply. Many studies have been done on detecting and quantifying microplastics in the environment and their impact on marine life. However, most studies exclude nanoscale particles from consideration, focusing instead on larger pieces amenable to visual inspection and common filtration techniques. Studies on microplastic accumulation in vivo tend to focus on large fish and sea mammals, and experimental models often use less-than-realistic methods of microplastic exposure (e.g., injection, spike-in, etc.). The focus of this project is to validate Fourier-Transform Infrared (FTIR) spectroscopy as an efficient, accessible tool for detecting and quantifying microplastics in fish tissue. FTIR was used to detect 0.5 um polystyrene particles in passively fed zebrafish larvae up to 7 days post-fertilization. Fluorescent microscopy and spectroscopy also revealed that the fluorescent polymer beads are taken up by the larvae. This accumulation was found to decrease survivability of the larvae up to 7 dpf.
- Notes:
- Thesis (Sc. M.)--Brown University, 2021
Citation
Maxwell, Tyler,
"Analysis of Polystyrene Microsphere Uptake in Passively Fed Danio rerio Larvae via FT-IR Spectroscopy and Fluorescence-Based Detection"
(2021).
Molecular Pharmacology, Physiology, and Biotechnology Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://repository.library.brown.edu/studio/item/bdr:jypputrk/
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Molecular Pharmacology, Physiology, and Biotechnology Theses and Dissertations
Theses and Dissertations for the Molecular Pharmacology, Physiology, and Biotechnology department....