Description
- Abstract:
- Human tissue regeneration is often characterized by the over-deposition of collagen, resulting in fibrotic scarring. Traditional research into wound healing and fibrosis relies heavily on the use of animal models, which are not only costly and time-consuming, but may not adequately mimic the human biological environment. A 3D ring-shaped microtissue model featuring highly-aligned collagen-dense tissue, previously developed by the Morgan Lab, has now been evaluated for its potential as an in vitro model of human extracellular matrix regeneration following tensile damage. By utilizing Collagen Hybridizing Peptide (CHP) to quantify collagen degradation, this research established a timeline of repair in 3D ring tissues, demonstrating the capacity of fibroblasts to regenerate the extracellular matrix independent of immune system influence. Additionally, modulation of the Hippo pathway successfully increased cellular proliferation following the induction of a wound, highlighting a promising area for further exploration in this model.
- Notes:
- Thesis (Sc. M.)--Brown University, 2024
Citation
Marx, Madeleine Claire,
"Evidence for Fibroblast-Directed Extracellular Matrix Remodeling Independent of Immune System Influence in a 3D Ring Microtissue Model"
(2024).
Biotechnology, Biology and Medicine Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://repository.library.brown.edu/studio/item/bdr:85bap9ab/
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Biology and Medicine Theses and Dissertations
Theses and Dissertations for the Biology and Medicine department....